Integrated analyses of the methylome and transcriptome to unravel sex differences in the perirenal fat from suckling lambs.

ABSTRACT: In sheep, differences were observed regarding fat accumulation and fatty acid (FA) composition between males and females, which may impact the quality and organoleptic characteristics of the meat. The integration of different omics technologies is a relevant approach for investigating biological and genetic mechanisms associated with complex traits. Here, the perirenal tissue of six male and six female Assaf suckling lambs was evaluated using RNA sequencing and whole-genome bisulfite sequencing (WGBS). A multiomic discriminant analysis using multiblock (s)PLS-DA allowed the identification of 314 genes and 627 differentially methylated regions (within these genes), which perfectly discriminate between males and females. These candidate genes overlapped with previously reported QTLs for carc

SUBMITTER: Fonseca PAS 

PROVIDER: S-EPMC9663842 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature