Label-free quantitative proteomic analysis of adult Drosophila heads.
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ABSTRACT:
SUBMITTER: Zhang Y
PROVIDER: S-EPMC9664018 | biostudies-literature | 2022 Dec
REPOSITORIES: biostudies-literature
Publications
Label-free quantitative proteomic analysis of adult <i>Drosophila</i> heads.
Zhang Yan Y Nezis Ioannis P IP
STAR protocols 20221111 4
LIR motif-containing proteins (LIRCPs) bind to LDS (LIR motif docking site) of Atg8-family proteins. In this protocol, we describe steps to identify <i>Drosophila</i> LIRCPs, in Atg8a LDS mutants we have created, via label-free quantitative proteomic analysis. We detail steps for extraction of proteins from adult <i>Drosophila</i> heads, followed by liquid chromatography-mass spectrometry (LC-MS/MS) analysis. We also describe screening steps of upregulated proteins in Atg8a LDS mutants, leading ...[more]
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