The Oral Microbiome and Lung Cancer Risk: An Analysis of 3 Prospective Cohort Studies.

ABSTRACT:

Background

Previous studies suggested associations between the oral microbiome and lung cancer, but studies were predominantly cross-sectional and underpowered.

Methods

Using a case-cohort design, 1306 incident lung cancer cases were identified in the Agricultural Health Study; National Institutes of Health-AARP Diet and Health Study; and Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial. Referent subcohorts were randomly selected by strata of age, sex, and smoking history. DNA was extracted from oral wash specimens using the DSP DNA Virus Pathogen kit, the 16S rRNA gene V4 region was amplified and sequenced, and bioinformatics were conducted using QIIME 2. Hazard ratios and 95% confidence intervals were calculated using weighted Cox proportional hazards models.

SUBMITTER: Vogtmann E 

PROVIDER: S-EPMC9664178 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature