Dual-labeled nanoparticles based on small extracellular vesicles for tumor detection.

ABSTRACT:

Background

Small extracellular vesicles (sEVs) are emerging natural nanoplatforms in cancer diagnosis and therapy, through the incorporation of signal components or drugs in their structure. However, for their translation into the clinical field, there is still a lack of tools that enable a deeper understanding of their in vivo pharmacokinetics or their interactions with the cells of the tumor microenvironment. In this study, we have designed a dual-sEV probe based on radioactive and fluorescent labeling of goat milk sEVs.

Results

The imaging nanoprobe was tested in vitro and in vivo in a model of glioblastoma. In vitro assessment of the uptake of the dual probe in different cell populations (RAW 264.7, U87, and HeLa) by optical and nuclear techniques (gamma counter, confoca

SUBMITTER: Santos-Coquillat A 

PROVIDER: S-EPMC9664624 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature