Synchronized Cardiac Impulses Emerge From Heterogeneous Local Calcium Signals Within and Among Cells of Pacemaker Tissue.

ABSTRACT:

Objectives

This study sought to identify subcellular Ca²⁺ signals within and among cells comprising the sinoatrial node (SAN) tissue.

Background

The current paradigm of SAN impulse generation: 1) is that full-scale action potentials (APs) of a common frequency are initiated at 1 site and are conducted within the SAN along smooth isochrones; and 2) does not feature fine details of Ca²⁺ signaling present in isolated SAN cells, in which small subcellular, subthreshold local Ca²⁺ releases (LCRs) self-organize to generate cell-wide APs.

Methods

Immunolabeling was combined with a novel technique to detect the occurrence of LCRs and AP-induced Ca²⁺ transients (APCTs) in individual pixels (chronopix) across the entire mouse SAN imag

SUBMITTER: Bychkov R 

PROVIDER: S-EPMC9665107 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature