Reversible and Irreversible Effects of Electroporation on Contractility and Calcium Homeostasis in Isolated Cardiac Ventricular Myocytes.

ABSTRACT:

Background

Irreversible electroporation is an energy form utilizing high-voltage pulsed electric field, leading to cellular homeostasis disruption and cell death. Recently, irreversible electroporation has shown promising results for the treatment of cardiac arrhythmias. However, reversible and irreversible effects of pulsed electric field on cardiac myocytes remain poorly understood. Here, we evaluated the influence of a monophasic single electric pulse (EP) on the contractility, Ca²⁺ homeostasis and recovery of cardiac myocytes.

Methods

Isolated rat left ventricular myocytes were electroporated using single monophasic EP of different durations and voltages. Sarcomere length and intracellular Ca²⁺ were simultaneously monitored for up to 20 minutes afte

SUBMITTER: Chaigne S 

PROVIDER: S-EPMC9665944 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature