Identification of novel linear epitopes in P72 protein of African swine fever virus recognized by monoclonal antibodies.

ABSTRACT: African swine fever (ASF) is one of the highly contagious and lethal diseases among domestic pigs and wild boars. The capsid protein P72 of African swine fever virus (ASFV) is very important for the diagnosis and vaccine development. However, the epitope of the protein is not clear. In this study, capsid protein P72 was expressed in Sf9 cells along with its chaperone B602L. A total of ten monoclonal antibodies (mAbs) specific to P72 protein were developed by fusions between SP2/0 cells and spleen cells of mice immunized with the recombinant-P72&B602L proteins expressed in Sf9 cells. Four linear B cell epitopes ³¹SNIKNVNKSY⁴⁰, ⁴¹GKPDP⁴⁵, ⁵⁶HLVHFNAH⁶³ and ¹⁸⁵ERLYE¹⁸⁹ were identified. Biological informatio

SUBMITTER: Yin D 

PROVIDER: S-EPMC9666363 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature