Microfluidic dose-response platform to track the dynamics of drug response in single mycobacterial cells.

ABSTRACT: Preclinical analysis of drug efficacy is critical for drug development. However, conventional bulk-cell assays statically assess the mean population behavior, lacking resolution on drug-escaping cells. Inaccurate estimation of efficacy can lead to overestimation of compounds, whose efficacy will not be confirmed in the clinic, or lead to rejection of valuable candidates. Time-lapse microfluidic microscopy is a powerful approach to characterize drugs at high spatiotemporal resolution, but hard to apply on a large scale. Here we report the development of a microfluidic platform based on a pneumatic operating principle, which is scalable and compatible with long-term live-cell imaging and with simultaneous analysis of different drug concentrations. We tested the platform with mycobacterial ce

SUBMITTER: Mistretta M 

PROVIDER: S-EPMC9666435 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature