High-affinity chromodomains engineered for improved detection of histone methylation and enhanced CRISPR-based gene repression.

ABSTRACT: Histone methylation is an important post-translational modification that plays a crucial role in regulating cellular functions, and its dysregulation is implicated in cancer and developmental defects. Therefore, systematic characterization of histone methylation is necessary to elucidate complex biological processes, identify biomarkers, and ultimately, enable drug discovery. Studying histone methylation relies on the use of antibodies, but these suffer from lot-to-lot variation, are costly, and cannot be used in live cells. Chromatin-modification reader domains are potential affinity reagents for methylated histones, but their application is limited by their modest affinities. We used phage display to identify key residues that greatly enhance the affinities of Cbx chromodomains for methy

SUBMITTER: Veggiani G 

PROVIDER: S-EPMC9666628 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature