Validation of suitable reference genes by various algorithms for gene expression analysis in Isodon rubescens under different abiotic stresses.

ABSTRACT: Isodon rubescens (Hemsley) H. Hara (Lamiaceae) is a traditional Chinese medicine plant that has been used to treat various human diseases. Oridonin is one of the main active ingredients, and the route of its molecular biosynthesis remains to be determined. The study of gene expression patterns can provide clues toward the understanding of its biological functions. The selection of suitable reference genes for normalizing target gene expression is the first steps in any quantitative real-time PCR (RT-qPCR) gene expression study. Therefore, validation of suitable reference genes is necessary for obtaining reliable results in RT-qPCR analyses of I. rubescens. Here, 12 candidate reference genes were chosen, and their expression stability in different tissues of I. rubescens and in leaves under

SUBMITTER: Lian C 

PROVIDER: S-EPMC9666634 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature