A genotyping by sequencing approach can disclose Apis mellifera population genomic information contained in honey environmental DNA.

ABSTRACT: Awareness has been raised over the last years on the genetic integrity of autochthonous honey bee subspecies. Genomic tools available in Apis mellifera can make it possible to measure this information by targeting individual honey bee DNA. Honey contains DNA traces from all organisms that contributed or were involved in its production steps, including the honey bees of the colony. In this study, we designed and tested a genotyping by sequencing (GBS) assay to analyse single nucleotide polymorphisms (SNPs) of A. mellifera nuclear genome using environmental DNA extracted from honey. A total of 121 SNPs (97 SNPs informative for honey bee subspecies identification and 24 SNPs associated with relevant traits of the colonies) were used in the assay to genotype honey DNA, which derives from thous

SUBMITTER: Bovo S 

PROVIDER: S-EPMC9666642 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature