Using cryoEM and cryoET to visualize membrane penetration of a non-enveloped virus.

ABSTRACT: Key to cell entry by non-enveloped viruses is virus-cell interactions at the cell or endosomal membrane. Here, we detail our protocols to capture such interactions between non-enveloped virus bluetongue virus (BTV) and vesicular membrane by cryogenic electron microscopy (cryoEM) and tomography (cryoET). Key steps include virus isolation, liposome preparation, virus-liposome incubation and vitrification, cryoEM and cryoET imaging, data processing for 3D reconstruction, and subtomogram averaging. The protocols can be generally applicable to studies of cell entry by other non-enveloped viruses. For complete details on the use and execution of this protocol, please refer to Xia et al. (2021).

SUBMITTER: Xia X 

PROVIDER: S-EPMC9667302 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature