Ultrahigh resolution MS¹/MS²-based reconstruction of metabolic networks in mammalian cells reveals changes for selenite and arsenite action.

ABSTRACT: Metabolic networks are complex, intersecting, and composed of numerous enzyme-catalyzed biochemical reactions that transfer various molecular moieties among metabolites. Thus, robust reconstruction of metabolic networks requires metabolite moieties to be tracked, which cannot be readily achieved with mass spectrometry (MS) alone. We previously developed an Ion Chromatography-ultrahigh resolution-MS¹/data independent-MS² method to track the simultaneous incorporation of the heavy isotopes ¹³C and ¹⁵N into the moieties of purine/pyrimidine nucleotides in mammalian cells. Ultrahigh resolution-MS¹ resolves and counts multiple tracer atoms in intact metabolites, while data independent-tandem MS (MS²) determines isotopic enrichmen

SUBMITTER: Fan TW 

PROVIDER: S-EPMC9667311 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature