Phosphorylation-mediated PI3K-Art signalling pathway as a therapeutic mechanism in the hydrogen-induced alleviation of brain injury in septic mice.

ABSTRACT: Our previous studies illustrated that 2% H₂ inhalation can protect against sepsis-associated encephalopathy (SAE) which is characterized by high mortality and has no effective treatment. To investigate the underlying role of protein phosphorylation in SAE and H₂ treatment, a mouse model of sepsis was constructed by caecal ligation and puncture (CLP), then treated with H₂ (CLP + H₂ ). Brain tissues of the mice were collected to be analysed with tandem mass tag-based quantitative proteomics coupled with IMAC enrichment of phosphopeptides and LC-MS/MS analysis. In proteomics and phosphoproteomics analysis, 268 differentially phosphorylated proteins (DPPs) showed a change in the phosphorylated form in the CLP + H₂ group (p < 0.05). Gene on

SUBMITTER: Bai Y 

PROVIDER: S-EPMC9667523 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature