Selection of a PD-1 blocking antibody from a novel fully human phage display library.

ABSTRACT: Programmed cell death protein 1 (PD-1) is an immunoregulatory target which is recognized by different monoclonal antibodies, approved for the therapy of multiple types of cancer. Different anti-PD-1 antibodies display different therapeutic properties and there is a pharmaceutical interest to generate and characterize novel anti-PD-1 antibodies. We screened multiple human antibody phage display libraries to target novel epitopes on the PD-1 surface and we discovered a unique and previously undescribed binding specificity (termed D12) from a new antibody library (termed AMG). The library featured antibody fragments in single-chain fragment variable (scFv) format, based on the IGHV3-23*03 (V_H ) and IGKV1-39*01 (Vκ) genes. The D12 antibody was characterized by surface plasmon resona

SUBMITTER: Peissert F 

PROVIDER: S-EPMC9667898 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature