Intrapericardial Exosome Therapy Dampens Cardiac Injury via Activating Foxo3.

ABSTRACT:

Background

Mesenchymal stem cell (MSC)-derived exosomes are well recognized immunomodulating agents for cardiac repair, while the detailed mechanisms remain elusive. The Pericardial drainage pathway provides the heart with immunosurveillance and establishes a simplified model for studying the mechanisms underlying the immunomodulating effects of therapeutic exosomes.

Methods

Myocardial infarction (MI) models with and without pericardiectomy (corresponding to Tomy MI and NonTomy MI) were established to study the functions of pericardial drainage pathway in immune activation of cardiac-draining mediastinal lymph node (MLN). Using the NonTomy MI model, MSC exosomes or vehicle PBS was intrapericardially injected for MI treatment. Via cell sorting and RNA-seq (RNA-sequencing) ana

SUBMITTER: Zhu D 

PROVIDER: S-EPMC9667926 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature