Genome-wide quantification of contributions to sexual fitness identifies genes required for spore viability and health in fission yeast.

ABSTRACT: Numerous genes required for sexual reproduction remain to be identified even in simple model species like Schizosaccharomyces pombe. To address this, we developed an assay in S. pombe that couples transposon mutagenesis with high-throughput sequencing (TN-seq) to quantitatively measure the fitness contribution of nonessential genes across the genome to sexual reproduction. This approach identified 532 genes that contribute to sex, including more than 200 that were not previously annotated to be involved in the process, of which more than 150 have orthologs in vertebrates. Among our verified hits was an uncharacterized gene, ifs1 (important for sex), that is required for spore viability. In two other hits, plb1 and alg9, we observed a novel mutant phenotype of poor spore health wherein viab

SUBMITTER: Billmyre RB 

PROVIDER: S-EPMC9668190 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature