A two-step process for in silico screening to assess the performance of qRTPCR kits against variant strains of SARS-CoV-2.

ABSTRACT:

Background

Since inception of the COVID-19 pandemic, early detection and isolation of positive cases is one of the key strategies to restrict disease transmission. Real time reverse transcription polymerase chain reaction (qRTPCR) has been the mainstay of diagnosis. Most of the qRTPCR kits were designed against the target genes of original strain of SARS-CoV-2. However, with the emergence of variant strains of SARS-CoV-2, sensitivity of the qRTPCR assays has reportedly reduced. In view of this, it is critical to continuously monitor the performance of the qRTPCR kits in the backdrop of variant strains of SARS-CoV-2. Real world monitoring of assay performance is challenging. Therefore, we developed a two-step in-silico screening process for evaluating the performance of various qRTP

SUBMITTER: Gupta S 

PROVIDER: S-EPMC9668695 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature