Histone H2B.8 compacts flowering plant sperm through chromatin phase separation.

ABSTRACT: Sperm chromatin is typically transformed by protamines into a compact and transcriptionally inactive state^1,2. Sperm cells of flowering plants lack protamines, yet they have small, transcriptionally active nuclei with chromatin condensed through an unknown mechanism^3,4. Here we show that a histone variant, H2B.8, mediates sperm chromatin and nuclear condensation in Arabidopsis thaliana. Loss of H2B.8 causes enlarged sperm nuclei with dispersed chromatin, whereas ectopic expression in somatic cells produces smaller nuclei with aggregated chromatin. This result demonstrates that H2B.8 is sufficient for chromatin condensation. H2B.8 aggregates transcriptionally inactive AT-rich chromatin into phase-separated condensates, which facilitates nuclear compaction without reduc

SUBMITTER: Buttress T 

PROVIDER: S-EPMC9668745 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature