Spatial genomics maps the structure, nature and evolution of cancer clones.

ABSTRACT: Genome sequencing of cancers often reveals mosaics of different subclones present in the same tumour^1-3. Although these are believed to arise according to the principles of somatic evolution, the exact spatial growth patterns and underlying mechanisms remain elusive^4,5. Here, to address this need, we developed a workflow that generates detailed quantitative maps of genetic subclone composition across whole-tumour sections. These provide the basis for studying clonal growth patterns, and the histological characteristics, microanatomy and microenvironmental composition of each clone. The approach rests on whole-genome sequencing, followed by highly multiplexed base-specific in situ sequencing, single-cell resolved transcriptomics and dedicated algorithms to link these l

SUBMITTER: Lomakin A 

PROVIDER: S-EPMC9668746 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature