Ontology highlight
ABSTRACT:
SUBMITTER: Saunders A
PROVIDER: S-EPMC9668842 | biostudies-literature | 2022 Nov
REPOSITORIES: biostudies-literature
Saunders Arpiar A Huang Kee Wui KW Vondrak Cassandra C Hughes Christina C Smolyar Karina K Sen Harsha H Philson Adrienne C AC Nemesh James J Wysoker Alec A Kashin Seva S Sabatini Bernardo L BL McCarroll Steven A SA
Nature communications 20221116 1
Brain function depends on synaptic connections between specific neuron types, yet systematic descriptions of synaptic networks and their molecular properties are not readily available. Here, we introduce SBARRO (Synaptic Barcode Analysis by Retrograde Rabies ReadOut), a method that uses single-cell RNA sequencing to reveal directional, monosynaptic relationships based on the paths of a barcoded rabies virus from its "starter" postsynaptic cell to that cell's presynaptic partners. Thousands of th ...[more]