Quantitative PET imaging of the CD4 pool in nonhuman primates.

ABSTRACT:

Purpose

Previous SPECT and PET semi-quantitative in vivo imaging studies in monkeys have demonstrated specific uptake of radiolabeled rhesus recombinant anti-CD4 monoclonal antibody fragment CD4R1-F(ab΄)₂ in the spleen and clusters of lymph nodes (LNs) but yielded conflicting results of imaging the gut CD4 + T-cell pool. Here, using PET dynamic imaging with kinetic analysis, we performed a fully quantitative CD4 imaging in rhesus macaques.

Methods

The biodistributions of [⁸⁹Zr]Zr-CD4R1-F(ab΄)₂ and/or of [⁸⁹Zr]Zr-ibalizumab were performed with static PET scans up to 144 h (6 days) post-injection in 18 rhesus macaques with peripheral blood CD4 + T cells/μl ranging from ~ 20 to 2400. Fully quantitative analysis with a 4-h dynamic scan

SUBMITTER: Kim I 

PROVIDER: S-EPMC9668939 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature