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Au@Ag nanostructures for the sensitive detection of hydrogen peroxide.

ABSTRACT: Hydrogen peroxide (H₂O₂) is an important molecule in biological and environmental systems. In living systems, H₂O₂ plays essential functions in physical signaling pathways, cell growth, differentiation, and proliferation. Plasmonic nanostructures have attracted significant research attention in the fields of catalysis, imaging, and sensing applications because of their unique properties. Owing to the difference in the reduction potential, silver nanostructures have been proposed for the detection of H₂O₂. In this work, we demonstrate the Au@Ag nanocubes for the label- and enzyme-free detection of H₂O₂. Seed-mediated synthesis method was employed to realize the Au@Ag nanocubes with high uniformity. The Au@Ag nanocubes were demonstrated to exhibit the ability to monitor the H₂O₂ at concentration levels lower than 200 µM with r² = 0.904 of the calibration curve and the limit of detection (LOD) of 1.11 µM. In the relatively narrow range of the H₂O₂ at concentration levels lower than 40 µM, the LOD was calculated to be 0.60 µM with r² = 0.941 of the calibration curve of the H₂O₂ sensor. This facile fabrication strategy of the Au@Ag nanocubes would provide inspiring insights for the label- and enzyme-free detection of H₂O_2.

SUBMITTER: Yeh IH

PROVIDER: S-EPMC9668984 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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Au@Ag nanostructures for the sensitive detection of hydrogen peroxide.

Yeh I-Hsiu IH Tadepalli Sirimuvva S Liu Keng-Ku KK

Scientific reports 20221116 1

Hydrogen peroxide (H2O2) is an important molecule in biological and environmental systems. In living systems, H2O2 plays essential functions in physical signaling pathways, cell growth, differentiation, and proliferation. Plasmonic nanostructures have attracted significant research attention in the fields of catalysis, imaging, and sensing applications because of their unique properties. Owing to the difference in the reduction potential, silver nanost ...[more]

PMID: 36385155

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Project description:Thiol proteins are important in cellular antioxidant defenses and redox signalling. It is postulated that reactive oxidants cause selective thiol oxidation, but relative sensitivities of different cell proteins and critical targets are not well characterized. We exposed Jurkat cells to H2O2 for 10 min and measured changes in reversibly oxidized proteins by labelling with iodoacetamidofluorescein and two-dimensional electrophoresis. At 200 microM H2O2, which caused activation of the MAP (mitogen-activated protein) kinase ERK (extracellular-signal-regulated kinase), growth arrest and apoptosis, relatively few changes were seen. A total of 28 spots were reversibly oxidized (increased labelling intensity) and 24 decreased. The latter included isoforms of peroxiredoxins 1 and 2, which were irreversibly oxidized. Oxidation of GAPDH (glyceraldehyde-3-phosphate dehydrogenase) was striking, and other affected proteins included glutathione S-transferase P1-1, enolase, a regulatory subunit of protein kinase A, annexin VI, the mitotic checkpoint serine/threonine-protein kinase BUB1beta, HSP90beta (heat-shock protein 90beta) and proteosome components. At 20 microM H2O2, changes were fewer, but GAPDH and peroxiredoxin 2 were still modified. Dinitrochlorobenzene treatment, which inhibited cellular thioredoxin reductase and partially depleted GSH, caused reversible oxidation of several proteins, including thioredoxin 1 and peroxiredoxins 1 and 2. Most changes were distinct from those with H2O2, and changes with H2O2 were scarcely enhanced by dinitrochlorobenzene. Relatively few proteins, including deoxycytidine kinase, nucleoside diphosphate kinase and a proteosome activator subunit, responded only to the combined treatment. Thus most of the effects of H2O2 were not linked to thioredoxin oxidation. Our study has identified peroxiredoxin 2 and GAPDH as two of the most oxidant-sensitive cell proteins and has highlighted how readily peroxiredoxins undergo irreversible oxidation.

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Au@Ag nanostructures for the sensitive detection of hydrogen peroxide.

Publications

Au@Ag nanostructures for the sensitive detection of hydrogen peroxide.

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