Multiomics analysis couples mRNA turnover and translational control of glutamine metabolism to the differentiation of the activated CD4⁺ T cell.

ABSTRACT: The ZFP36 family of RNA-binding proteins acts post-transcriptionally to repress translation and promote RNA decay. Studies of genes and pathways regulated by the ZFP36 family in CD4⁺ T cells have focussed largely on cytokines, but their impact on metabolic reprogramming and differentiation is unclear. Using CD4⁺ T cells lacking Zfp36 and Zfp36l1, we combined the quantification of mRNA transcription, stability, abundance and translation with crosslinking immunoprecipitation and metabolic profiling to determine how they regulate T cell metabolism and differentiation. Our results suggest that ZFP36 and ZFP36L1 act directly to limit the expression of genes driving anabolic processes by two distinct routes: by targeting transcription factors and by targeting transcripts en

SUBMITTER: Matheson LS 

PROVIDER: S-EPMC9669047 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature