The Metabolome Weakens RNA Thermodynamic Stability and Strengthens RNA Chemical Stability.

ABSTRACT: We examined the complex network of interactions among RNA, the metabolome, and divalent Mg²⁺ under conditions that mimic the Escherichia coli cytoplasm. We determined Mg²⁺ binding constants for the top 15 E. coli metabolites, comprising 80% of the metabolome by concentration at physiological pH and monovalent ion concentrations. These data were used to inform the development of an artificial cytoplasm that mimics in vivo E. coli conditions, which we term "Eco80". We empirically determined that the mixture of E. coli metabolites in Eco80 approximated single-site binding behavior toward Mg²⁺ in the biologically relevant free Mg²⁺ range of ∼0.5 to 3 mM Mg²⁺, using a Mg²⁺-sensitive fluorescent

SUBMITTER: Sieg JP 

PROVIDER: S-EPMC9669196 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature