The single-cell atlas of cultured human endometrial stromal cells.

ABSTRACT:

Objective

To systematically analyze the cell composition and transcriptome of primary human endometrial stromal cells (HESCs) and transformed human endometrial stromal cells (THESCs).

Design

The primary HESCs from 3 different donors and 1 immortalized THESC were collected from the human endometrium at the midsecretory phase and cultured in vitro.

Setting

Academic research laboratory.

Patient(s)

None.

Intervention(s)

None.

Main outcome measure(s)

Single-cell ribonucleic acid sequencing analysis.

Result(s)

We found the individual differences among the primary HESCs and bigger changes between the primary HESCs and THESCs. Cell clustering with or without integration identified cell clusters belonging to mature, proliferative, and active fibrobl

SUBMITTER: Li R 

PROVIDER: S-EPMC9669198 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature