MCO1 and MCO3, two putative ascorbate oxidases with ferroxidase activity, new candidates for the regulation of apoplastic iron excess in Arabidopsis.

ABSTRACT: Iron (Fe) is an essential metal ion that plays a major role as a cofactor in many biological processes. The balance between the Fe²⁺ and Fe³⁺ forms is central for cellular Fe homeostasis because it regulates its transport, utilization, and storage. Contrary to Fe³⁺ reduction that is crucial for Fe uptake by roots in deficiency conditions, ferroxidation has been much less studied. In this work, we have focused on the molecular characterization of two members of the MultiCopper Oxidase family (MCO1 and MCO3) that share high identity with the Saccharomyces cerevisiae ferroxidase Fet3. The heterologous expression of MCO1 and MCO3 restored the growth of the yeast fet3fet4 mutant, impaired in high and low affinity Fe uptake and otherwi

SUBMITTER: Brun A 

PROVIDER: S-EPMC9669615 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature