AMPK/MFF Activation: Role in Mitochondrial Fission and Mitophagy in Dry Eye.

ABSTRACT:

Purpose

To assess the role of mitochondrial morphology and adenosine monophosphate-activated protein kinase (AMPK)/mitochondrial fission factor (MFF) in dry eye and the underlying mechanisms.

Methods

Immortalized human corneal epithelial cells (HCECs) and primary HCECs were cultured under high osmotic pressure (HOP). C57BL/6 female mice were injected subcutaneously with scopolamine. Quantitative real-time PCR was used to measure mRNA expression. Protein expression was assessed by western blot and immunofluorescence staining. Mitochondrial morphology was observed by confocal microscopy and transmission electron microscopy.

Results

First, HOP induced mitochondrial oxidative damage to HCECs, accompanied by mitochondrial fission and increased mitophagy. Then, AMPK/MFF pat

SUBMITTER: Peng F 

PROVIDER: S-EPMC9669805 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature