Optimization and Characterization of a Liposomal Azithromycin Formulation for Alternative Macrophage Activation.

ABSTRACT: Liposomal azithromycin (L-AZM) promotes macrophage polarization toward an M2-like phenotype in the context of myocardial infarction that results in improved cardiovascular outcomes in mice. To improve upon this formulation, we sought to identify optimized formulation, stability, and biological activity parameters necessary to enhance the immunomodulatory activity and efficacy of L-AZM. While our parent formulation contains a mixture of long-chain saturated phosphatidylcholine and phosphatidylglycerol lipids, we evaluated a series of formulations with different amounts of unsaturated lipids and cholesterol with the goal of improving the loading capacity and stability of the formulations. We also introduce fusogenic lipids to improve the cytosolic delivery to enhance the immune modulatory pr

SUBMITTER: Masud AA 

PROVIDER: S-EPMC9670256 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature