Albumin alleviated esketamine-induced neuronal apoptosis of rat retina through downregulation of Zn²⁺-dependent matrix metalloproteinase 9 during the early development.

ABSTRACT:

Aims

Esketamine upregulates Zn²⁺-dependent matrix metalloproteinase 9 (MMP9) and increases the neuronal apoptosis in retinal ganglion cell layer during the early development. We aimed to test whether albumin can alleviate esketamine-induced apoptosis through downregulating Zn²⁺-dependent MMP9.

Methods

We investigate the role of Zn²⁺ in esketamine-induced neuronal apoptosis by immunofluorescence. MMP9 protein expression and enzyme activity were investigated by zymography in situ., western blot and immunofluorescence. Whole-mount retinas from P7 Sprague-Dawley rats were used.

Results

We demonstrated that esketamine exposure increased Zn²⁺ in the retinal GCL during the early development. Zn²⁺-dependent MMP9 expressio

SUBMITTER: Zhang K 

PROVIDER: S-EPMC9670403 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature