High-throughput interspecies profiling of acidic plant hormones using miniaturised sample processing.

ABSTRACT:

Background

Acidic phytohormones are small molecules controlling many physiological functions in plants. A comprehensive picture of their profiles including the active forms, precursors and metabolites provides an important insight into ongoing physiological processes and is essential for many biological studies performed on plants.

Results

A high-throughput sample preparation method for liquid chromatography-tandem mass spectrometry determination of 25 acidic phytohormones classed as auxins, jasmonates, abscisates and salicylic acid was optimised. The method uses a small amount of plant tissue (less than 10 mg fresh weight) and acidic extraction in 1 mol/L formic acid in 10% aqueous methanol followed by miniaturised purification on reverse phase sorbent accommodated in pipet

SUBMITTER: Siroka J 

PROVIDER: S-EPMC9670418 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature