Precursor prioritization for p-cymene production through synergistic integration of biology and chemistry.

ABSTRACT: The strategy of synergistic application of biological and chemical catalysis is an important approach for efficiently converting renewable biomass into chemicals and fuels. In particular, the method of determining the appropriate intermediate between the two catalytic methods is critical. In this work, we demonstrate p-cymene production through the integration of biosynthesis and heterogenous catalysis and show how a preferred biologically derived precursor could be determined. On the biological side, we performed the limonene and 1,8-cineole production through the mevalonate pathway. Titers of 0.605 g/L and a 1.052 g/L were achieved, respectively. This difference is in agreement with the toxicity of these compounds toward the producing microorganisms, which has implications for subsequent

SUBMITTER: Lin HH 

PROVIDER: S-EPMC9670573 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature