Dataset Information

Detection and characterization of ESBL-producing Escherichia coli and additional co-existence with mcr genes from river water in northern Thailand.

ABSTRACT:

Background

Extended-spectrum β-lactamase producing Escherichia coli (ESBL-producing E. coli) have emerged, causing human and animal infections worldwide. This study was conducted to investigate the prevalence and molecular genetic features of ESBL-producing and multidrug-resistant (MDR) E. coli in river water.

Methods

A total of 172 E. coli samples were collected from the Kok River and Kham River in Chiang Rai, Thailand, during a 10-month period (2020-2021).

Results

We detected 45.3% of E. coli to be MDR. The prevalence of ESBL-producers was 22%. Among those ESBL-producing strains, CTX-M-15 (44.7%) was predominantly found, followed by CTX-M-55 (26.3%), CTX-M-14 (18.4%), and CTX-M-27 (10.5%). The bla _TEM-1 and bla _TEM-116 genes were found to be co-harbored with the bla _CTX-M genes. Mobile elements, i.e., ISEcp1 and Tn3, were observed. Twelve plasmid replicons were found, predominantly being IncF (76.3%) and IncFIB (52.6%). Whole genome sequencing of ten selected isolates revealed the co-existence of ESBL with mcr genes in two ESBL-producing E. coli. A wide diversity of MLST classifications was observed. An mcr-1.1-pap2 gene cassette was found to disrupt the PUF2806 domain-containing gene, while an mcr-3.4 contig on another isolate contained the nimC/nimA-mcr-3.4-dgkA core segment.

Discussion

In conclusion, our data provides compelling evidence of MDR and ESBL-producing E. coli, co-existing with mcr genes in river water in northern Thailand, which may be disseminated into other environments and so cause increased risks to public health.

SUBMITTER: Chotinantakul K

PROVIDER: S-EPMC9671034 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Publications

Detection and characterization of ESBL-producing <i>Escherichia coli</i> and additional co-existence with <i>mcr</i> genes from river water in northern Thailand.

Chotinantakul Kamonnaree K Chusri Pattranuch P Okada Seiji S

PeerJ 20221114

<h4>Background</h4>Extended-spectrum β-lactamase producing <i>Escherichia coli</i> (ESBL-producing <i>E. coli</i>) have emerged, causing human and animal infections worldwide. This study was conducted to investigate the prevalence and molecular genetic features of ESBL-producing and multidrug-resistant (MDR) <i>E. coli</i> in river water.<h4>Methods</h4>A total of 172 <i>E. coli</i> samples were collected from the Kok River and Kham River in Chiang Rai, Thailand, during a 10-month period (2020-2 ...[more]

PMID: 36405029

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