Zinc Binding Inhibits Cellular Uptake and Antifungal Activity of Histatin-5 in Candida albicans.

ABSTRACT: Histatin-5 (Hist-5) is a polycationic, histidine-rich antimicrobial peptide with potent antifungal activity against the opportunistic fungal pathogen Candida albicans. Hist-5 can bind metals in vitro, and metals have been shown to alter the fungicidal activity of the peptide. Previous reports on the effect of Zn²⁺ on Hist-5 activity have been varied and seemingly contradictory. Here, we present data elucidating the dynamic role Zn²⁺ plays as an inhibitory switch to regulate Hist-5 fungicidal activity. A novel fluorescently labeled Hist-5 peptide (Hist-5*) was developed to visualize changes in internalization and localization of the peptide as a function of metal availability in the growth medium. Hist-5* was verified for use as a model peptide and retained anti

SUBMITTER: Campbell JX 

PROVIDER: S-EPMC9671271 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature