Unsaturated fatty acid synthesis in Enterococcus faecalis requires a specific enoyl-ACP reductase.

ABSTRACT: The Enterococcus faecalis genome contains two enoyl-ACP reductases genes, fabK and fabI, which encode proteins having very different structures. Enoyl-ACP reductase catalyzes the last step of the elongation cycle of type II fatty acid synthesis pathway. The fabK gene is located within the large fatty acid synthesis operon whereas fabI is located together with two genes fabN and fabO required for unsaturated fatty acid synthesis. Prior work showed that FabK is weakly expressed due to poor translational initiation and hence virtually all the cellular enoyl ACP reductase activity is that encoded by fabI. Since FabK is a fully functional enzyme, the question is why FabI is an essential enzyme. Why not increase FabK activity? We report that overproduction of FabK is lethal whereas FabI overprod

SUBMITTER: Dong H 

PROVIDER: S-EPMC9671860 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature