Septin-5 and -7-IgGs: Neurologic, Serologic, and Pathophysiologic Characteristics.

ABSTRACT:

Background and objectives

We sought to determine clinical significance of neuronal septin autoimmunity and evaluate for potential IgG effects.

Methods

Septin-IgGs were detected by indirect immunofluorescence assays (IFAs; mouse tissue and cell based) or Western blot. IgG binding to (and internalization of) extracellular septin epitopes were evaluated for by live rat hippocampal neuron assay. The impact of purified patient IgGs on murine cortical neuron function was determined by recording extracellular field potentials in a multielectrode array platform.

Results

Septin-IgGs were identified in 23 patients. All 8 patients with septin-5-IgG detected had cerebellar ataxia, and 7 had prominent eye movement disorders. One of 2 patients with co-existing septin-7-IgG had addi

SUBMITTER: Hinson SR 

PROVIDER: S-EPMC9672904 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature