Detection of Treponema pallidum in whole blood samples of patients with syphilis by the polymerase chain reaction.

ABSTRACT: Syphilis is caused by the bacterium Treponema pallidum. The diagnosis is based on clinical data and serological analysis; however, the sensitivity and specificity of such tests may vary depending on the type of test and stage of the infection. In order to overcome this premise, this study utilized the polymerase chain reaction (PCR) for the detection of T. pallidum DNA in whole blood samples of patients with syphilis. The blood samples from patients with or without symptoms of syphilis, but with positive results in enzyme-linked immunosorbent assay (ELISA), were included in this study. A venereal disease research laboratory (VDRL) test was performed for all collected sera samples. For PCR, the T. pallidum DNA was extracted from the collected blood samples and a specific primer set was desi

SUBMITTER: Queiroz JHFS 

PROVIDER: S-EPMC9673146 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature