Heterosubstituted Derivatives of PtPFPP for O₂ Sensing and Cell Analysis: Structure-Activity Relationships.

ABSTRACT: Biological applications of phosphorescent probes for sensing molecular oxygen (O₂) and bioimaging have gained popularity, but their choice is rather limited. We describe a family of new heterosubstituted phosphorescent bioprobes based on the Pt(II)-tetrakis(pentafluorophenyl)porphyrin (PtPFPP) dye. The probes are produced by simple click modification of its para-fluorine atoms with thiols, such as 1/2-thio-glucose, thio-poly(ethylene glycol) (PEG), or cysteamine. The probes were designed to have one cell-targeting moiety and three polar moieties forming a hydrophilic shell. Their chemical synthesis and purification were optimized to produce high reaction yields and easy scale-up. The ability to perform as cell-permeable or -impermeable probes was tuned by the polarity and

SUBMITTER: Zanetti C 

PROVIDER: S-EPMC9673148 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature