HNRNPA2B1-mediated m6A modification of TLR4 mRNA promotes progression of multiple myeloma.

ABSTRACT:

Background

Multiple myeloma (MM) is a malignancy of plasma cells that remains incurable. Toll-like receptor 4 (TLR4) acts as a stress-responsive signal, protecting mitochondria during proteasome inhibitor (PI) exposure, maintaining mitochondrial metabolism and increasing drug resistance in MM. However, the mechanism of TLR4 regulation remains elusive.

Aims

The purpose of this study was to investigate the methylation pattern of multiple myeloma and its effect on the expression of HNRNPA2B1 and downstream targets.

Methods

The methylation level in MM and normal bone marrow specimens was detected using a colorimetric assay. HNRNPA2B1 gene knockdown was achieved in RPMI 8226 MM cells via adenovirus transfection. CCK8 and flow cytometric assays were used to detect prolifera

SUBMITTER: Jia C 

PROVIDER: S-EPMC9673362 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature