TRF-3013b inhibits gallbladder cancer proliferation by targeting TPRG1L.

ABSTRACT:

Background

tRNA-derived fragments (tRFs) are newly discovered noncoding RNAs and regulate tumor progression via diverse molecular mechanisms. However, the expression and biofunction of tRFs in gallbladder cancer (GBC) have not been reported yet.

Methods

The expression of tRFs in GBC was detected by tRF and tiRNA sequencing in GBC tissues and adjacent tissues. The biological function of tRFs was investigated by cell proliferation assay, clonal formation assay, cell cycle assay, and xenotransplantation model in GBC cell lines. The molecular mechanism was discovered and verified by transcriptome sequencing, fluorescence in situ hybridization (FISH), target gene site prediction, and RNA binding protein immunoprecipitation (RIP).

Results

tRF-3013b was significantly downreg

SUBMITTER: Zou L 

PROVIDER: S-EPMC9673407 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature