Dataset Information

Case report: Mixed infection of bovine papillomaviruses associated with squamous papilloma of the upper alimentary tract in a dairy cow.

ABSTRACT: Bovine papillomavirus (BPV) infection can induce neoplastic lesions in both cutaneous and mucosal epithelia in cattle. This study describes the BPV types associated with proliferative lesions with diverse histopathological features present in the upper alimentary tract of a dairy cow suffering from chronic diarrhea from Midwestern Brazil. At autopsy, warts and plaques composed of multiple spherical nodules were observed in the esophageal mucosa, the areas surrounding and constricting the opening of the cardia, and the rumen pillars. One esophageal papillomatous proliferative lesion and a smooth-surfaced proliferative lesion located at the rumen entrance were evaluated by histopathological and molecular analyses. PCR amplification of partial fragments of the BPV L1 and E1 genes was performed followed by sequencing of the obtained amplicons. Upon histopathological evaluation, the esophageal lesion was classified as a squamous papilloma, whereas the other ruminal proliferative lesion consisted of a fibropapilloma. Direct sequencing of PCR products obtained from ruminal fibropapilloma DNA revealed the presence of BPV2. Sequencing of inserts from selected clones containing partial fragments of the BPV L1 and E1 genes revealed a mixed infection of BPV types 2 and 4 in the esophageal squamous papilloma. The findings reported in our investigation reinforce the association of BPV with benign lesions of the bovine alimentary tract in both single and mixed infections, as previously demonstrated to occur in a buffalo. In addition, this report represents the documentation of the occurrence of massive alimentary papillomatosis associated with BPV types 2 and 4 in cattle raised on lands without infestation by bracken fern in Midwestern Brazil.

SUBMITTER: Fernandes KP

PROVIDER: S-EPMC9673478 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Case report: Mixed infection of bovine papillomaviruses associated with squamous papilloma of the upper alimentary tract in a dairy cow.

Fernandes Kamila Peruchi KP Alfieri Amauri Alcindo AA Darold Gabriela Molinari GM Boabaid Fabiana Marques FM Dall Agnol Alais Maria AM Lunardi Michele M

Frontiers in veterinary science 20221104

Bovine papillomavirus (BPV) infection can induce neoplastic lesions in both cutaneous and mucosal epithelia in cattle. This study describes the BPV types associated with proliferative lesions with diverse histopathological features present in the upper alimentary tract of a dairy cow suffering from chronic diarrhea from Midwestern Brazil. At autopsy, warts and plaques composed of multiple spherical nodules were observed in the esophageal mucosa, the areas surrounding and constricting the opening ...[more]

PMID: 36406071

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Project description:In the present study bacterial communities from both, the gastrointestinal and respiratory tract of pre-weaned dairy calves fed two different milk-feeding programs were characterized using 16S rRNA gene sequencing. Twenty female Holstein calves (38.8 ± 1.40 kg of BW) were fed pasteurized waste milk (pWM) containing residues of various antimicrobials. Twenty additional calves (38.1 ± 1.19 kg of BW) were fed milk replacer (MR) with similar nutrient composition (27.5% crude protein, 32.1% fat) compared to waste milk (28.6% crude protein, 30.0% fat) from day 1 to weaning at day 49 of study. Fecal samples and nasal swabs were collected on day 42 only from calves that were not treated with therapeutic antibiotics throughout the study, which were 8 MR and 10 pWM calves. To assess the impact of the two feeding regimes on the fecal and nasal microbiota, α and β-diversity measures were calculated, and the relative abundance of operational taxonomic units (OTUs) at different taxonomic levels was determined for each sample. In general, Chao1, PD Whole Tree, and Shannon diversity indices were similar for the fecal and nasal bacterial communities of calves regardless of the feeding regime. However, principal coordinate analysis based on unweighted Unifrac distances indicated differences in the structure of bacterial communities of calves fed milk replacer compared with those from calves fed pasteurized waste milk. The relative abundance of the Streptococcaceae family and the genus Histophilus was greater (P < 0.05) in the nasal microbiota of calves fed milk replacer than in those fed pasteurized waste milk. However, the genus Prevotella tended (P = 0.06) to be more relatively abundant in the respiratory tract of calves fed pasteurized waste milk than in those fed milk replacer. Differences in relative abundances of bacterial taxa in gut microbiota were only observed at the phylum level, suggesting that antimicrobial residues present in waste milk have a non-specific influence at a lower taxonomical level.

Dataset Information

Case report: Mixed infection of bovine papillomaviruses associated with squamous papilloma of the upper alimentary tract in a dairy cow.

Publications

Case report: Mixed infection of bovine papillomaviruses associated with squamous papilloma of the upper alimentary tract in a dairy cow.

Similar Datasets

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