Improved microbial genomes and gene catalog of the chicken gut from metagenomic sequencing of high-fidelity long reads.

ABSTRACT:

Background

Due to the importance of chicken production and the remarkable influence of the gut microbiota on host health and growth, tens of thousands of metagenome-assembled genomes (MAGs) have been constructed for the chicken gut microbiome. However, due to the limitations of short-read sequencing and assembly technologies, most of these MAGs are far from complete, are of lower quality, and include contaminant reads.

Results

We generated 332 Gb of high-fidelity (HiFi) long reads from the 5 chicken intestinal compartments and assembled 461 and 337 microbial genomes, of which 53% and 55% are circular, at the species and strain levels, respectively. For the assembled microbial genomes, approximately 95% were regarded as complete according to the "RNA complete" criteria, which

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC9673493 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature