Pointed-end processive elongation of actin filaments by Vibrio effectors VopF and VopL.

ABSTRACT: According to the cellular actin dynamics paradigm, filaments grow at their barbed ends and depolymerize predominantly from their pointed ends to form polar structures and do productive work. We show that actin can elongate at the pointed end when assisted by Vibrio VopF/L toxins, which act as processive polymerases. In cells, processively moving VopF/L speckles are inhibited by factors blocking the pointed but not barbed ends. Multispectral single-molecule imaging confirmed that VopF molecules associate with the pointed end, actively promoting its elongation even in the presence of profilin. Consequently, VopF/L can break the actin cytoskeleton's polarity by compromising actin-based cellular processes. Therefore, actin filament design allows processive growth at both ends, which sug

SUBMITTER: Kudryashova E 

PROVIDER: S-EPMC9674292 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature