Resistance to aztreonam-avibactam due to CTX-M-15 in the presence of penicillin-binding protein 3 with extra amino acids in Escherichia coli.

ABSTRACT: Aztreonam-avibactam is a promising combination to treat carbapenem-resistant Enterobacterales including coverage for metallo-β-lactamases. Escherichia coli strains resistant to aztreonam-avibactam have emerged but resistance mechanisms remain to be elucidated. We performed a study to investigate the mechanism for aztreonam-avibactam in a carbapenem-resistant Escherichia coli clinical strain. This strain was resistant to aztreonam-avibactam (aztreonam MIC, 16 mg/L in the presence of 4 mg/L avibactam). Whole genome sequencing revealed that the strain carried metallo-β-lactamase gene bla _NDM-4 and the extended-spectrum β-lactamase (ESBL) gene bla _CTX-M-15 and had a YRIK four amino acid insertion in penicillin-binding protein 3 (PBP3). bla</

SUBMITTER: Ma K 

PROVIDER: S-EPMC9674307 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature