Selection of red fluorescent protein for genetic labeling of mitochondria and intercellular transfer of viable mitochondria.

ABSTRACT: The phenomenon of intercellular mitochondrial transfer has attracted great attention in various fields of research, including stem cell biology. Elucidating the mechanism of mitochondrial transfer from healthy stem cells to cells with mitochondrial dysfunction may lead to the development of novel stem cell therapies to treat mitochondrial diseases, among other advances. To visually evaluate and analyze the mitochondrial transfer process, dual fluorescent labeling systems are often used to distinguish the mitochondria of donor and recipient cells. Although enhanced green fluorescent protein (EGFP) has been well-characterized for labeling mitochondria, other colors of fluorescent protein have been less extensively evaluated in the context of mitochondrial transfer. Here, we generated differe

SUBMITTER: Taiko I 

PROVIDER: S-EPMC9674635 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature