Observation of hapten-induced sensitization responses for the development of a mouse skin sensitization test, including the elicitation phase.

ABSTRACT: The only official method that can detect the skin sensitizing potential of chemicals, including the elicitation response, is the OECD test guideline (TG) 406. However, this guideline uses guinea pigs, which requires complex procedures. Since a simple and complete test method for evaluating skin sensitization is needed, especially for mechanistic studies of skin sensitization, this study confirmed the reactivity of mice to skin sensitizing substances. We set up a protocol involving one induction exposure of the test substance to the back skin, followed by three challenge exposures to the auricle (Protocol 2), and compared their skin sensitization responses with the results of two exposures to the auricle and back skin every 2 weeks (Protocol 1) and a local lymph node assay (TG442B). A hapte

SUBMITTER: Kamata R 

PROVIDER: S-EPMC9674688 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature