Comparison of two isolation methods of tobacco-derived extracellular vesicles, their characterization and uptake by plant and rat cells.

ABSTRACT: Plant extracellular vesicles (pEVs) derived from numerous edible sources gain a lot of attention in recent years, mainly due to the potential to efficiently carry bioactive molecules into mammalian cells. In the present study, we focus on isolation of PDNVs (plant-derived nanovesicles) and pEVs from callus culture and from BY-2 culture of Nicotiana tabacum (tobacco). Tobacco was selected as a source of plant vesicles, as it is commonly used by human, moreover it is a model organism with established techniques for cultivation of explant cultures in vitro. Explant cultures are suitable for the isolation of pEVs in large quantities, due to their fast growth in sterile conditions. As the efficiency of isolation methods varies, we were comparing two methods of isolation. We evaluated biophysica

SUBMITTER: Kocholata M 

PROVIDER: S-EPMC9674704 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature