Genome characterization and CRISPR-Cas9 editing of a human neocentromere.

ABSTRACT: The maintenance of genome integrity is ensured by proper chromosome inheritance during mitotic and meiotic cell divisions. The chromosomal counterpart responsible for chromosome segregation to daughter cells is the centromere, at which the spindle apparatus attaches through the kinetochore. Although all mammalian centromeres are primarily composed of megabase-long repetitive sequences, satellite-free human neocentromeres have been described. Neocentromeres and evolutionary new centromeres have revolutionized traditional knowledge about centromeres. Over the past 20 years, insights have been gained into their organization, but in spite of these advancements, the mechanisms underlying their formation and evolution are still unclear. Today, through modern and increasingly accessible genome ed

SUBMITTER: Palazzo A 

PROVIDER: S-EPMC9674717 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature