Understanding the separation of timescales in bacterial proteasome core particle assembly.

ABSTRACT: The 20S proteasome core particle (CP) is a molecular machine that is a key component of cellular protein degradation pathways. Like other molecular machines, it is not synthesized in an active form but rather as a set of subunits that assemble into a functional complex. The CP is conserved across all domains of life and is composed of 28 subunits, 14 α and 14 β, arranged in four stacked seven-member rings (α₇β₇β₇α₇). While details of CP assembly vary across species, the final step in the assembly process is universally conserved: two half proteasomes (HPs; α₇β₇) dimerize to form the CP. In the bacterium Rhodococcus erythropolis, experiments have shown that the formation of the HP is completed within minutes, while the dimeri

SUBMITTER: Itagi P 

PROVIDER: S-EPMC9674962 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature