Coexpression Network Analysis-Based Identification of Critical Genes Differentiating between Latent and Active Tuberculosis.

ABSTRACT:

Methods

Three Gene Expression Omnibus (GEO) microarray datasets (GSE19491, GSE98461, and GSE152532) were downloaded, with GSE19491 and GSE98461 then being merged to form a training dataset. Hub genes capable of differentiating between ATB and LTBI were then identified through differential expression analyses and a WGCNA analysis of this training dataset. Receiver operating characteristic (ROC) curves were then used to gauge to the diagnostic accuracy of these hub genes in the test dataset (GSE152532). Gene expression-based immune cell infiltration and the relationship between such infiltration and hub gene expression were further assessed via a single-sample gene set enrichment analysis (ssGSEA).

Results

In total, 485 differentially expressed genes were analyzed, with the WG

SUBMITTER: Chen L 

PROVIDER: S-EPMC9674975 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature